Nine fluorescence parameter analysis on a four-color fluorescence activated flow cytometer
نویسندگان
چکیده
منابع مشابه
Color-blind fluorescence detection for four-color DNA sequencing.
We present an approach called pulsed multiline excitation (PME) for measurements of multicomponent, fluorescence species and demonstrate its application in capillary electrophoresis for DNA sequencing. To fully demonstrate the advantages of PME, a fluorescent dye set has been developed whose absorption maxima span virtually the entire visible spectrum. Unlike emission wavelength-dependent appro...
متن کاملSimultaneous Fluorescence and Impedance Micro Cytometer – a Modular System
Cytometry is used to identify cells in a variety of fields such as haematology and environmental monitoring. We describe a modular micro-fabricated cytometer that uses on-chip methods to simultaneously excite fluorescence and measure the electrical impedance of individual particles. The integration of impedance and fluorescence cytometry on a single chip provides essentially the same informatio...
متن کاملFluorescence incoherent color holography.
We present a new imaging method to record multicolor digital holograms from objects emitting fluorescent light. The fluorescent light specific to the emission wavelength of various fluorescent dyes after excitation of three dimensional (3D) objects is recorded on a digital monochrome camera after reflection from a diffractive optical element (DOE). For each wavelength of fluorescent emission, t...
متن کاملImproved diffuse fluorescence flow cytometer prototype for high sensitivity detection of rare circulating cells in vivo.
Detection and enumeration of rare circulating cells in mice are important problems in many areas of preclinical biomedical research. Recently, we developed a new method termed "diffuse fluorescence flow cytometry" (DFFC) that uses diffuse photons to increase the blood sampling volume and sensitivity versus existing in vivo flow cytometry methods. In this work, we describe a new DFFC prototype w...
متن کاملHigh Throughput Fluorescence Based Flow Cytometer Using 3d Microfluidics for Parallel Sheath Flow Focusing and Embeded High N.a. Microlens
We report a high throughput microfluidic fluorescence based flow cytometer with parallel sheath flow focusing in 32 channels using only 2 inlets. A detection throughput of 7000 beads/sec in each channel and 224,000 beads/sec for 32 channels has been accomplished. This fluorescence flow cytometer is realized by utilizing 3D soft lithography microfabrication technique to achieve multilayer PDMS s...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Cytometry Part A
سال: 2006
ISSN: 1552-4922,1552-4930
DOI: 10.1002/cyto.a.20221